Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKHYFNQDRNFWINV---QQNDVLAVKNLKEEYGISDEMLTYSLDKNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTT-------PNTEYVIRLIRSLL-ERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLLQLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYR---KLSEKEK----EELDDALIEARQAVE-MTNLASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQ-LPFTHSVFGWGIALIISLVLSIWMLIALWRRIR 5JRW Chain:A ((83-370)) -------------WINITGIHRTDV--VQRVGEFFGIHPLVLERILNVHQRPKVEF--FENYVFIVLKMFTYDKNLHELESEQVSLILTKNCVLMFQEKIGDVFDPVRER-IRYNRGIIRKKRADYLLYSLID----ALVDDYFVLLEKIDDEIDVLEEEVLERPEKETVQRTHQLKRNLVEL----RKTIWPLREVLS-SLYRDVPPLIEKETVPYFRRVYDHTIQIADTVETFRDIVSGLLD----VYLSSVSNKTNEVMKVLTIIATIFMPLTFIAGIYGMNFEYMPELRWKWGYPVVLAVMGVIAVIMVVYFKKK--

General information: TITO was launched using: RESULT: Template: 5JRW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 975 9898 10.15 36.93 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 10.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_5JRW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JRW-query.scw PDB file : Tito_Scwrl_5JRW.pdb: