Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDGLGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRFTLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDTIVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGLRGSKASVNKFKPDLFFAVDCSAADDT--VTKNGTFGHLGEGTLMRIQD----PGLIMLPRLREYLLDIAETNNIPYQYFVSKGG-TDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDF-----------EAAREMLIQTLKGLDKSTVNTIVYGK 4WWV Chain:A ((10-365)) -----IELIKKLTSLHAPSGREDPVKDLVAELMKSHVDKLWIDVWGNVVGYRKGSKGSG-KIMIAAHMDEIGLFISHIEDDGFLRVIPIGGVLERTLLYQRVVVRTRDGRLYRGVIGLKPPHV----EAQKVPELRELFIDVGASSKEEVEKMGIRVGDIAV-FDREVAELGWNRITSKAFDDRVGVVVMLKALEMLEKHDVDVYLV--ATVQEEVGLKGAKTSAYGISPDVALAIDVTIASDVPGVAKSEWFTRLGYGPAIKIVDGRNAGGLIAHPKVGEFLVSIAEKKRIPYQLDVISGGTTDASTIALNKEGVAAGTISIPSRYIHSPVEVVDLRDLYNASLLAKAFIEEATPEWIQSIKGV------------

General information: TITO was launched using: RESULT: Template: 4WWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2021 -131733 -65.18 -394.41 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -65.18 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_4WWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WWV-query.scw PDB file : Tito_Scwrl_4WWV.pdb: