Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKFLDMSLVQRIVVGILVGILFGIFFPTW-------TFISILGTLFVSCLKAIAPILVF---FLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSEKA-PQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAF-----------RARSESTKELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEY------------LQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGV--IQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDCLDGLSER 2NWX Chain:A ((12-411)) ---------VLQKILIGLILGAIVGLILGHYGYAHAVHTYVKPFGDLFVRLLKMLVMPIVFASLVVGAASISPARLG-RVGVK-IVVYYLLTSAFAVTLGIIMARLFNPGAGIHLAVGGQQF----PPLVHILLDIVPTNPFGALANGQVLPTIFFAIILGIAITYLMNSENEKVRKSAETLLDAINGLAEAMYKIVNGVMQYAPIGVFALIAYVMAEQGVHVVGELAKVTAAVYVGLTLQILLVY-----FVLLKIYGIDPISFIKHA---------KDAMLTAFVTRSSSGTLPVTMRVAKEMGISEGIYSFTLPLGATINMDGTALYQGVCTFFIANALGSHLTVGQQLTIVLTAVLASIGTAGVPGAGAIMLAMVLHSVGLPLTDPNVAAAYAMILGIDAILDMGRTMVNVTGDLTGTAI-------------------------

General information: TITO was launched using: RESULT: Template: 2NWX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1588 -211481 -133.17 -589.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -133.17 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_2NWX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NWX-query.scw PDB file : Tito_Scwrl_2NWX.pdb: