Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNPEISVVPVPETEIKKITLKND--YLEVVLLNYGARLHQIFAPDKEGKSENVLLSY-DRFEDVLTDKSFFGATVGPVAGRIRDASW----GEHRLEKNCGSHHIHGGTKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASSLVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPT-GNWTKKEKLPIDFRKSPTLQEILACYPDGLD-DVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFSTTGFEADFS-VNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSAQ 4RNL Chain:A ((20-343)) -------------TRVDRWTLESGPAGLRVRVLTYGGIVQTVEAPDRDGMRGQLALGFADLASYAAHGGSYFGALVGRYANRIAGASFVLDGRTDALTPNNGRHSLHGGPGGFSRVVWDAR--EVDGGVQLHRVSPDGEEGFPGALDVRVTYTLSAGALRIVSCATTDAPTVVNLTNHTYLNLGGDGSGSAAGHELRLAASRYTPVDGTGIPVPGAPAEVTGTRFDFRAA---RAVAGAYDHNFALDGGVREAPR---TVAELYDPRSGRALALATTEPGLQLYTADHLDGTLTGTSGVPYGPAAGLALETQHFPDSPNRPDFPSTVLRPGESYRSETVYAFSVR

General information: TITO was launched using: RESULT: Template: 4RNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1939 46788 24.13 149.01 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 24.13 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_4RNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNL-query.scw PDB file : Tito_Scwrl_4RNL.pdb: