Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTVSVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDRIGALGQQGHLAEVKLGNEAA--YDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAPSTFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGD-DGT-NPSDIGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQTYKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEKFFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFDAASGSKTPKESADEAVKTISEAIEQKY 2ZYK Chain:A ((43-393)) --------------------------------------------------------------FTKKTGIQVEVVPVALLKQQEKLTLDGPAGKGADLVTWPHDRLGEAVTKGLLQPIQVDNSVKNQFDDVAMKALTYGGKLYGLPKAIESVALIYNKKLMGQVPATYDELFQYAKANNKPDEQK----YGVLFEANNFYYTYFLFAAKGAAVFKEQDGTLDPNEIGLNSPEAVQGMNEVQKWFTEA--RLPQSLKA--DTVNGLFKSGKVAAVINGPWAIKDYQAAGINVGVAPLPKID-GKDAQTFIGVKGWYLSAYSKYPKYATELMQFLTSKEALASRFKETGEIPPQKELLNDPMIKNNPVVNGFAKQASKGVPMPSIPEMGVVWEPINNAHTFVAQGKQTPEQALNDAVKIMKEKIQ---

General information: TITO was launched using: RESULT: Template: 2ZYK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1871 35526 18.99 102.38 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 18.99 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_2ZYK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYK-query.scw PDB file : Tito_Scwrl_2ZYK.pdb: