Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKSTSTTSITNTSTGN--------VDSQHSQSSIQSFSISSTSEM----QESTVPSLPLENSDKALLFLIAHRSELQSDDIVISFYQKIDRDYLFTASSKQ-----IRSQGGSGSVGFYRVSPEGSITMTDANGTPF 1O0Q Chain:A ((168-303)) -----------------------------------THEIGHTLGLSHPGD------NPTYRDAVYAEDTRAYSVMSYWSEKNTGQVFTKTGEGAYASAPLLDDIAAVQKLYGANLETRADDTVYGFNSTADRDFYSATSSTDKLIFSVWDGGGNDTLDFSGFSQNQKINLT-------

General information: TITO was launched using: RESULT: Template: 1O0Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 441 21070 47.78 186.46 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 47.78 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_1O0Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O0Q-query.scw PDB file : Tito_Scwrl_1O0Q.pdb: