Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQARKRILIGGGSEQPMRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSSTKTAS---TTEQTMATSNNEK----KTALDQLKEQQPNVPM-PL-DVPVSSGYLNIAATHTKQGY-SILYYRTDRP------LGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSRITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFGKITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL 5FQG Chain:A ((75-199)) -----------------------------------------------------TNSSPVNNVTLSSSDFINENGDLISSNNIKISWLKETLANIGRSNPSAPLEPFPDIIHNSGSLNIEKNKIASAWINIKIPRNAKPGIYNGSIEVTADELEK-----SYTFDYSF----EVLNLVQPLPSETNTQ--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FQG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 386 42375 109.78 388.76 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 109.78 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_5FQG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FQG-query.scw PDB file : Tito_Scwrl_5FQG.pdb: