Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFG-AGLVVCEMIS--DQGIHFR--NKKTLEMLHIEETEHP--LSLQIFGGNKDSLVE-AAQFVEENTAADIIDINMGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAV--NIPVTIKMRIGWDE-EHVFAVENALAAQSAGASAIAMHGRTRVQMYEGK-ADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWMLDYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKGEKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL 3W9Z Chain:A ((2-242)) --------------RVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWG--VDLNCGC-------------LLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAV--QQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIP--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1127 13091 11.62 63.86 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 11.62 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3W9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W9Z-query.scw PDB file : Tito_Scwrl_3W9Z.pdb: