Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFVIVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTFAGKKVKEQKKRWDEPSTLVRSVNYVSD 2ZNM Chain:A ((107-122)) -------------------------------------------------------------------VAGKWALSQKGFDGKK----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 12 833 69.42 52.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : 69.42 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.46 QMean score : -0.105

(partial model without unconserved sides chains): PDB file : Tito_2ZNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZNM-query.scw PDB file : Tito_Scwrl_2ZNM.pdb: