Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPRADLLLRRPISLNHIDHETNTCRIIYRV--EGEGTRVFSTLSAGDHLDVMGPLGNGFDLSNLHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGET--RIATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRMACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII 1EP2 Chain:B ((4-254)) --QEMMTVVSQREVAYNIFEMVLKGTLVDEMDLPGQFLHLAVPNGAMLLRRPISISSWDKRAKTCTILYRIGDETTGTYKLSKLESGAKVDVMGPLGNGFPVAEVTSTDKILIIGGGIGVPPLYELAKQLEKTGCQMTILLGFASENVKILENEFSNLKNVTLKIATDDGSYGTKGHVGMLMNEIDFEVDALYTCGAPAMLKAVAKKYDQLERLYISMESRMACGIGACYACVEHDKEDE--SHALKVCEDGPVF-------

General information: TITO was launched using: RESULT: Template: 1EP2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1326 -45940 -34.65 -185.99 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -34.65 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_1EP2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP2-query.scw PDB file : Tito_Scwrl_1EP2.pdb: