Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILLIRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVKVPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASEIEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD 5KND Chain:G ((13-213)) ------NPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILLIRKNNELRQAIEKETQTAMKDFVLAKSTVEEAFIDE------ENVSISVVEKNIMSVKVPLMNFQ-----------------NAELDRSIDGFTQLLPKLLKLAEVEKTCQLMAEEIEKTRRRVNALEYMTIPQLEETIYYIKMKLEENERAEVTRLIKVKNM----

General information: TITO was launched using: RESULT: Template: 5KND.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 402 -35214 -87.60 -197.83 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain G : 0.88 3D Compatibility (PKB) : -87.60 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_5KND.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KND-query.scw PDB file : Tito_Scwrl_5KND.pdb: