Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAASRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRGDISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQNTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVTTGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQVDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS 2MHE Chain:B ((128-159)) ----KVEFVGAYPSYDEAHKAWKAKAQATVDNAHAR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 42 -1434 -34.14 -44.81 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -34.14 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2MHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MHE-query.scw PDB file : Tito_Scwrl_2MHE.pdb: