Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KKGDCFMGLYEAGGRNPNHIIGQETPDDSGIFELRRENGSVIQV---------KVKKCQLVAYWGW--VPLGQELRV-FP----SVGYDAKR--KTAP-LPSLSPNRH-------SGQGLKVRGAN-TWIAF----------------------- 5TPM Chain:A ((1-155)) SNADLLETRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEAAHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAREASHRHLAFIEEILLDRSREESRRERSLRRLE

General information: TITO was launched using: RESULT: Template: 5TPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 307 -317 -1.03 -3.02 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -1.03 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.055

(partial model without unconserved sides chains): PDB file : Tito_5TPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TPM-query.scw PDB file : Tito_Scwrl_5TPM.pdb: