Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GPSGVKAESSVRNPKYGDHCELTVWIHYKGKDIPLMRYYVEIGG--DLTFDEKDGAWKHCVIKTTKQACQATWT---GGSKSEPCPPKNINVDARKVIQ--------QHEFESESDTWWKRDI---------------- 3ESM Chain:A ((2-139)) -SLHVTADAP--GAAQGGYSVVTFRVPTESETAATTAMTVTLPNVRSARTEPMPGWTARVDRNDKSEAVSVTWTADPGNPGVQPGQFQRFVVSIGPLPSAETVSFPAEQTYSDGRVVAWNQPPAASEPEHPAPTLTLAT

General information: TITO was launched using: RESULT: Template: 3ESM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 475 3547 7.47 33.14 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.47 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_3ESM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ESM-query.scw PDB file : Tito_Scwrl_3ESM.pdb: