Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------SSSDTDSDSSPDRGLSRMCCVYKIHPGG-NI-WSTKKGEQA-------------WFRRRFSKYEVM-----AYDRCNLE-WGFSGKPRGLTFEFLWDKEAAADGTC 1I4F Chain:B ((1-100)) MIQRTPKIQVYSRHPAENGKSNF-L--NCYVSGFHPSDIEVDL-LKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYA-CRVNHVTLS-QP--KIVK--WDRDM------

General information: TITO was launched using: RESULT: Template: 1I4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 215 14431 67.12 209.14 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : 67.12 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_1I4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I4F-query.scw PDB file : Tito_Scwrl_1I4F.pdb: