Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQALFGGGCFWCVEAVFLQIRGVEKVTSGYAGGHTTHPTYEQVCQGDTQHAEVVLIDFDEQQVTYSQLLDVFFATHDPTTLNRQGNDIGTQYRSVIYYFNEEQKQAAEHT---IQTLK-----DDDLDIVTELSPA-----PTFYPAEDYHQNYYEKNPSQGYCNFAIPPKLLKLYSKFQHLMKDQ 4D7L Chain:A ((63-232)) TETIYIGIGCYWGAEKLFWETPGVVYTSVGFAGGITPNPTYRETCTGRTNHTEIVEVVYDPTQVTFDELVVKAMEAHDPTQGYRQGNDTGTQYRSAIYTAGPNAEQQAQRAREIVEHYAPKLAAAGLGRITTEILPLASTPAGEYYMAEDEHQQYLHKNPL-GYCPHHSTG----------------

General information: TITO was launched using: RESULT: Template: 4D7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 819 -48389 -59.08 -308.21 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -59.08 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_4D7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D7L-query.scw PDB file : Tito_Scwrl_4D7L.pdb: