Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIALFYMGGTFGCIGEPLAPMPYDQFLPQLEKVIP--------------------------PHLTVDCFAAPNIVDSSACTAPDWLRLIQRIQQLQLEGYQHFVVIHGTDTLSYAAATLARFLGQ-SCHIVITGSQYPLLNIQGDNTREFTDAIENLYLALEQVIAL-LVGAYLAFHHQVFHAQTALKTHTTELDAFSGLSSNVEFTPQQNEL----I--V--QDAQI--EKAASFQLLNWMMQPIATQHLVQQLRHLLPAPPHFLVLQGFGTGNIAVNDEFLATLDELYTRGCVPILATQVTFGGIDQRYAISTWAKTAKIVINDAHSHADLYAKALQIYLKY-PTPEQWLNHWNENLH 4R8K Chain:C ((31-378)) ERHLLLIYTGGTLGMQSKGGVLVPGPGLVT-LLRTLPMFHDKEFAQAQGLPDHALALPPASHGPRVLYTVLECQPLLDSSDMTIDDWIRIAKIIER-HYEQYQGFVVIHGTDTMASGASMLSFMLENLHKPVILTGAQVPIRVLWN-------DARENLLGALLVAGQYIIPEVCLFMNSQLFRGNRVTKVDSQKFEAFCSPNLSPLATVGADVTIAWDLVRKVKWKDPLVVHSNMEHDVALLRLYPGIPASL---VRAFLQPPLKGVVLETFGSGNGPSKPDLLQELRAAAQRGLIMVNCSQCLRGSVTPGYAT--SLAGANIVSGLDMTSEAALAKLSYVLGLPELSLERRQELLAKDLR

General information: TITO was launched using: RESULT: Template: 4R8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1595 -76678 -48.07 -248.15 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -48.07 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4R8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R8K-query.scw PDB file : Tito_Scwrl_4R8K.pdb: