Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MFKGPDMQAIILDTETHTLNGLP---IEIAYAPIE-INAGKLTLDKSKLFDQLYQVGTPISYAAMAVHHILESDLEN---------QPHYKTFKLPDT-----TTYIIGHNIDYDIAAIARCGVD-------VSHIKPICTLALTRKTWPDAEAHNISALIYMISQGSSKA-RELLKGAHRADADIILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALE-SAF 2F96 Chain:A ((19-219)) RHPMARRFRGYLPVVVDVETGGFNSATDALLEIAATTVGMDEKGFLFPEHTYFFRIEPFEGANIEPAALEFTGIKLDHPLRMAVQEEAALTEIFRGIRKALKANGCKRAILVGHNSSFDLGFLNAAVARTGIKRNPFHPFSSFDTATLAGLAYGQTVLAKACQA---------AGMEFDNREAHSARYDTEKTAELFCGIVNRWK-------------------------------------------------------EMGGW

General information: TITO was launched using: RESULT: Template: 2F96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 763 -17278 -22.64 -101.64 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -22.64 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_2F96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F96-query.scw PDB file : Tito_Scwrl_2F96.pdb: