Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRIPVINTSHLDRIDELLVDNIDTGEFKLHRSVFTDEALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPIIIARNRNGELNAMMNACSHRGAQLCRYKRGNKATYTCPFHGWTFNNSGKLLKVKDPTDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLEEFLGETTKIIDMIVDQSEHGLEVL-RGSSTYTYEGNWKLTAENGA-DGYHVSAVHWNYAATTQHRKETQAAD-NIRAMSAGSWGKQGGGSYGFENGHMLLWTQW----AN---PEDRPNFPK-ADEYTEKYGEAMSKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPLSVNRTEVTIYCIAPKGEA---RARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIALEWNDMCRGSKHWIYGPDDAANEIGLKPMLSGIKT---EDEGLYLAQHQYWLKTMKHAIATEKEIADQGETA 2GBW Chain:E ((7-433)) ---------------------LVDTVNASQSRQVFWDEDVYALEIERIFSRAWLMLGHESLVPKPGDFITTYMAEDKVILSHQSDGTFRAFINSCSHRGNQICHADSGNAKAFVCNYHGWVFGQDGSLVDVPLES-RCYHNSLD-KQKLAAKSV-RVETYKGFIFGCHDPEAPSLEDYLGEFRYYLDTIWEGAGGGMELLGPP-MKSLLQCNWKVPAENFIGDGYHVGWTHAAALSQIGGELAGLAGNRADIPFDD------LGLQFTTRHGHGFGVIDNAAAGLHIKREGWTKFLEDTRGEVRRKFGPERERL-YLGHWNCSIFPNCSFLYGT-NTFKIWHPRGPHEIEVWTYTIVPRDADPATKSMIQREAIRTFGTAGTLESDDGENMSSATYINRGV---ITRNGRMNSTMGVGYEGP---HPVYP---GIVGISFIGETSYRGFYRFWKEMIDAPDWASVKAND-----

General information: TITO was launched using: RESULT: Template: 2GBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2352 -69862 -29.70 -170.40 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain E : 0.72 3D Compatibility (PKB) : -29.70 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_2GBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GBW-query.scw PDB file : Tito_Scwrl_2GBW.pdb: