Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEIIQPNEEIRITDGSKVDLHFSVAIENGVEIDNTRSR-------------EEPVSLTIGDGNLLPGFEKALLGLRAGDRRTVHLPPEDAFGPWNPENIQTFDTVKFEQ-R--PIPGHMIEFEDKAKATLFGVVKSVNDDITEIDFNHPLAGKNITFEVEIFKVTPAGQQGIKIM 3PRB Chain:B ((2-159)) ------------VEKGKMVKISYDGYV-DGKLFDTTNEELAKKEGIYNPAMIYGPVAIFAGEGQVLPGLDEAILEMDVGEEREVVLPPEKAFGKRDPSKIKLIPLSEFTKRGIKPIKGLTITIDG-----IPGKIVSINSGRVLVDFNHELAGKEVKYRIKIEEVVDDKKNIVKEI

General information: TITO was launched using: RESULT: Template: 3PRB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 552 26631 48.24 187.54 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 48.24 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_3PRB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PRB-query.scw PDB file : Tito_Scwrl_3PRB.pdb: