Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQHQSLFNKSQWTQLVKHFSAVAVLGLTIAAPAWAIDPTAKELRIGFQ--KS--SINFAIAKQQKLFEQEFPNAKITWNEFPAGPQILEALAVGSLDVGVTGDTPPVYAQAAGKPLYYIAYEAAKPLASAILVPKN--SQLKQLKDIKGKRIALQ-KGSSSHYLLVQAVRKAGLKWSDITPIWLT-PADARAAFQKGAVDAWAIWDPYYASAQLEDQARVLVSGK---------GLSPNYTFYLAAPNFVKQYPKAVPGLIKQINQADKWVQSY-QVETASAIGQST-GLKPATSDLFIKRRPRPSSAAPLNSKVIAEQQQIADIFTQQG-II-PKPISIKQAVWGAK 3QSL Chain:A ((31-334)) --------------------------------------EKAKVQIAVGGKPLIYYLPLTIAEVKGFFKDE--GLDVSIADFAGGSKALQAVVGGSADVVSGAFEHTLSLQAKGQFYRAFALQG-RAPMIGVGVSKKNLPGYKGPADLKGRKIGVTAPGSSTNMVVNFFLAKHGLKASDVSFIGVGAGAGAVTALRSGQIDAISNTDPVVSMLETSGDIQIIVDTRTLKDTKEIFGGNMPAGCLYAPQAFVDANPNTAQALTNAIVRADKWIQKAGADEIAKAVPEGYLLGDPAVYKAAIGKSMEG--LSPDGVIPEDGAATALKALAAFVPDFDAAKVDPAKAWTNE-

General information: TITO was launched using: RESULT: Template: 3QSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1796 26981 15.02 95.34 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 15.02 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_3QSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QSL-query.scw PDB file : Tito_Scwrl_3QSL.pdb: