Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYQMITREQCLYWDQEDELKKFKDEFALPEGVIYLDGNSLGARPKKSLAVAQHIISQEWGEDLINSWN-KADWWGLPTRLGDKVAKLVGAEQGEVVISDSTTLNLFKVLSAAVKIQADKFPEHKIIVAEKDAFPTDIYIIEGFIDLIQKGYQVELID--------GVE-DLSRALEKDVAVVVLSHVNYRTGYFYDMASINEQIHSK----DALVIWDLCHSVGAVPMHLNQTDSDFAIGCTYKYLNGGPGSPALLWVNEKHRDQFWQPLSGWWSHK-KPFDMAQHYEPANSIRRYLCGTQPVISMSLIECGVDIFLHADM-QKIREKSLKLTDLFIQLVHQECSEFGFELITPLDHKHRGSHVSYRHEF---GYEIIQALIARGVIGDYRE-PAVLRFGITPLYLGFEDIWNAVQHLKATMLNSEWKNKEYLVRGEVT 1ELU Chain:A ((10-389)) -------------------------QFPGLANKTYFNFGGQGILPTVALEAIT-AMYGYLQENGPFSIAANQHIQQLIAQLRQALAETFNVDPNTITITDNVTTGCDIVLWGLDWH------QGDEILLTDCEHPGIIAIVQAIAARFGI--TYRFFPVAATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEIMAVCRRHQGNYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGPAGV-GGLYIHGDCLGEINPTYVGWRSITYGAKGE--PTGWAEGGKRFEVATSAYPQYAGLLAALQLHQRQGTAEERYQAICQRSEFLWRGLNQL---PHVHCLATSA--PQAGLVSFTVDSPLGHRAIVQKLEEQRIYLRTIADPDCIRACCHY-ITDEEEINHLLARLADFG-----------------

General information: TITO was launched using: RESULT: Template: 1ELU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1949 -20806 -10.68 -57.79 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -10.68 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1ELU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ELU-query.scw PDB file : Tito_Scwrl_1ELU.pdb: