Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQTPEALTTEQFKQAIIDKG-QYYH--I-YHPFHVMMYEGKATQQQIQAWVANRYYYQINIPLKDAAIMANCPD--QRVRQEWIQRMIDQDGEYPDGGGREAWLRLAEAVGLSREQVISEELVLPGVRFAVDAYVNFARRASWREAASSSL-TELFAPQIHQSRLESWPQHYPWIDDKGYEYFRSRLSQARRDVEHGLTITLDSFTTHEQQQRMLEILQFKLDILWSILDALTLAYVHNEAPYHSVTQERVWHKGLFK 3DDE Chain:B ((4-226)) ----------IIDLTKLEQKVATMWDSILTNSPFIHEVLDGKATKALYAIYMTETYHYTKHNAKNQALVGIMGKDLPGKYLSFCFHHAHEEA------GHELMALSDIASIGFDREDVLSS-KPLPATETLIAYLYWISATGNPVQRLGYSYWAENVYGYI-DPVLKAIQSTLD-LTPQSMKFFIAHSKIDAKHAEEVNEMLHEVCKTQEDVDSVVAVMENSLVLTARILDDVWKEYQLFQS-----------------

General information: TITO was launched using: RESULT: Template: 3DDE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 924 -22900 -24.78 -106.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -24.78 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_3DDE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DDE-query.scw PDB file : Tito_Scwrl_3DDE.pdb: