Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKATE--YIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASERVD--------VE--------------------VEKKHPFDFVLWKHAKENEPSW---ASPWGNGRP---GWHIECS---------AMSTCCL-GNHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWIHVGFINVDGEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSS-HYRSPVNFSDVALKEAKT--SLTRFYHSFKAYQQVYGQT-----------TTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 2CT8 Chain:B ((6-421)) -----------------------KFYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELGISPKELVDRNAERFKKLWEFLKIE-YTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGE-YE-------------------------------KEPSYFFRLSKYQDKLLELYEKNPEFIQPDYRRNEIISFVKQGLK-DLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDKVEIYWPADLHLVGKDILRFHTVYWPAFLMSL-GYELPKKVFAHGWWTVEGKKMSKTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSRVVNMAHKFLGGEVSGARDEEYAKIAQESIKNYENYME-KVNFYKAIEEILKFTSYLNKYVDEKQP------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2CT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1571 -29271 -18.63 -90.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -18.63 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2CT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CT8-query.scw PDB file : Tito_Scwrl_2CT8.pdb: