Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLFDFVKGIGKKNTAPAEPQAAPATPAEPSAQEIANKLLGLIKSLGLGVEGLSVTYNGSTDTAIIKGQVQSQADKEKIILIVGNVDHVAQVDDQMTVATPEPESKFYTVKSGDNLSKIAKEFYGDANQYQKIFEANKPMLKDPDEIFPGQVLRIPQ 5FIM Chain:A ((1-148)) MGLFNFVKDAGEKLWDAVTGQHDK--------DDQAKKVQEHLNKTGIPDADKVN-IQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVKSGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPE

General information: TITO was launched using: RESULT: Template: 5FIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 784 -42929 -54.76 -290.06 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -54.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_5FIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIM-query.scw PDB file : Tito_Scwrl_5FIM.pdb: