Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIVTHGYFITEKIEDFLK 5FMF Chain:L ((28-57)) --KYICAECSSKLSLS---R-------TDAVRCKDCGHRILL-----------------

General information: TITO was launched using: RESULT: Template: 5FMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 77 -7759 -100.77 -258.63 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain L : 0.62 3D Compatibility (PKB) : -100.77 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_5FMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FMF-query.scw PDB file : Tito_Scwrl_5FMF.pdb: