Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDI--PVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPLHAVDFLAD-YFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLKAITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVE-TQGISRGQTLVDRYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 1Q8F Chain:D ((4-299)) -RKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD---KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTR-QAESTHAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGT-GNFTPSAEFNIFADPEAARVVFTS-GVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKVGITIDTDWFW-------------------------

General information: TITO was launched using: RESULT: Template: 1Q8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1896 -55430 -29.24 -189.83 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -29.24 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_1Q8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q8F-query.scw PDB file : Tito_Scwrl_1Q8F.pdb: