Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------PEFCAYFTGSNKSRRQVGVVRIGEIDTIIADGTELVVHAQDSRCQVILANGKPGPEWLSADPV 1EDQ Chain:A ((444-516)) YGRGWTGVNGYQNNIPFTGTATGP----VK-GTW-----ENGIVDYRQIAGQFMSG-EWQYTYDATAEAPYVFKPSTG-DLITFD--

General information: TITO was launched using: RESULT: Template: 1EDQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 471 3.68 9.61 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 3.68 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_1EDQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EDQ-query.scw PDB file : Tito_Scwrl_1EDQ.pdb: