Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------KCNISIL-KDGKYFTQKQEIAGWTVKF------HDL--DGNYVGKCKNAHTYACTYVTCEDLAPGYSP-GKSKDV---VASGNVGSL--GHDPSSSSPSSP------------------------------------------------ 1B93 Chain:A ((1-148)) MELTTRTLPARKHIALVAHDHC----KQMLMSWVERHQPLLEQHVLYATGTTGNLISRATGMN---VNA--MLSG--PMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDVKALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLA

General information: TITO was launched using: RESULT: Template: 1B93.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 234 4730 20.21 63.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 20.21 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.125

(partial model without unconserved sides chains): PDB file : Tito_1B93.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B93-query.scw PDB file : Tito_Scwrl_1B93.pdb: