Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---STSTKVKLENRKEPALCHVVIIKPAQIRHPLDTWVETFAWVKAGKVVEWHSPKY----GSVKVK--TDEDD----CHLTFLVNELINGYTCQLSR------------- 1IRA Chain:Y ((105-207)) YNAQAIFKQKLPVAGD----GGLVCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTY----LGKQYPITRVIEFITLEENKPT

General information: TITO was launched using: RESULT: Template: 1IRA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 237 5646 23.82 73.32 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain Y : 0.63 3D Compatibility (PKB) : 23.82 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_1IRA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IRA-query.scw PDB file : Tito_Scwrl_1IRA.pdb: