Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------QCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVAICKNAS---PVHCNYLKCTNLAAGFSAGTSTDVLSSGTVGSIGNDPQAQRQ 1UFF Chain:A ((1-93)) GSSGSSGYRALYP--FEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPC--NYVEKMPSSENEKAVSPKKALLPPTV-SLSATSGPSSG

General information: TITO was launched using: RESULT: Template: 1UFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 5382 20.23 65.63 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 20.23 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_1UFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFF-query.scw PDB file : Tito_Scwrl_1UFF.pdb: