Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCAAQMPPLAHIFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFATIHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEY-KETTEESIKETERFVSEMLQKNYSRVKPIVTPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTN-KTVMAHGCYLSAEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGYSYSMLDAIRRAVMVSNILLINK-VNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV 2I9U Chain:B ((9-427)) --------NLKIFKGNLIFTKTSDKFTIMKDSYIVVI-DGKIASVSSNLPDK--------YKGNPIIDFRNNIIIPGMNDLHAHASQYKNLGIGMDKELLPWLNNYTFPEEAKFLNVDYAKKTYGRLIKDLIKNGTTRVALFATLHKDSTIELFNMLIKSGIGAYVGKVNMDYN--CPDYLTENYITSLNDTEEIILKYKD-KSNIVKPIITPRFVPSCSNELMDGLGKLSYKYRLPVQSHLSENLDEIAVVKSLHKKSNFYGEVYDKFGLFGNTPTLMAHCIHSSKEEINLIKRNNVTIVHCPTSNFNLGSGMMPVRKYLNLGINVVLGSDISAGHTCSLFKVIAYAIQNSKIKWQESGKKDMFLSTSEAFYMATKKGGSFFG---KVGSFEEGYDFDALVINDSNLYP-ED--------YDLTERLERFIYLGDDRNIMKRYVCGNEIF------

General information: TITO was launched using: RESULT: Template: 2I9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2631 -89080 -33.86 -214.13 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -33.86 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2I9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9U-query.scw PDB file : Tito_Scwrl_2I9U.pdb: