Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETESILGLGG--KVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA---DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEPFILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVP------SASWIAAGDYHHHLAVNEWRGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDA--PIKWM-------TSSKLEITDSDGIVTRIRLAR 4Z6O Chain:B ((10-271)) --APAPDILRCAYAELVVTDLAKSRNFYVDVLGLHVSYEDENQIYLRSFEEFIHHNLVLTK-G-----PVAALKAMAFRVRTPEDVDKAEAYYQELGCRTERRKDGFVKGIGDALRVEDPLGFPYEFFFETTHVER-L-HM--------RY------------DLYSAGELVRLDHFNQVTPDVPRGRKYLED-LGFRVTEDIQDDEGTTYAAWMHRKGTVEDTALTG----------GNGPRLHHVAFSTHEKHNIIQICDKMGALRISDRIERGPGRHGVSNAFYLYILDPDNHRIEIYTQ-

General information: TITO was launched using: RESULT: Template: 4Z6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1270 -19699 -15.51 -81.40 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -15.51 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_4Z6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z6O-query.scw PDB file : Tito_Scwrl_4Z6O.pdb: