Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLSIAISSYNAAAYLHYCVESLVI--GGEQVGILIINDGSQDQTQEIAECLASKYPNIVRAIYQEN-KCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKILETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQYTMMHSLIYRTDLLRASQF 2Z86 Chain:C ((93-230)) -DGLSIVIPTYNRAKILAITLACLCNQKTIYDYEVIVADDGSKENIEEIVREFESL-LNIKYVRQKDYGYQLCAVRNLGLRAAKYNYVAILDCDMAPNPLWVQSYMELLAV----DDNVALIGPRKY-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Z86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 560 -25326 -45.22 -214.62 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -45.22 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_2Z86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z86-query.scw PDB file : Tito_Scwrl_2Z86.pdb: