Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRHISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANKAFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDGAGGVLLEASEQEHFLAES-LNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVFDFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMMEYGNTSAASIPILLSECVEQGLI-PLDGSQTVLLSGFGGGLTWGTLILTI 5KP2 Chain:B ((4-318)) -CYAEITGWGKCLPPATLSNHDLSTFLDTSDEWIQSRTGIEQRRISHV-NTSDLATVAAQHAIACAGVSVEEIDLIIVATCSPDSLIPNIASRVQQNLGIPSAAAFDLNAAATGFLYGLETATRLMQASHYRHALVIGAERLSFYLDWTKRDTAVLFGDGAGAVVLSKTEQKVGLQDAQIGCDAQGRDILAVPKFGTAMDRFDADNGYWAFDFVGKEIFKRAVRGMGAAAQQVLARSGLSTEEIDVVIPHQANIRIIQTLCDLAGIAQDKAFVNIHRYGNTSAATVPIALCEALEQGKIKPHD---DLLVAAFGAGLTWG------

General information: TITO was launched using: RESULT: Template: 5KP2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1893 -151403 -79.98 -483.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -79.98 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_5KP2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KP2-query.scw PDB file : Tito_Scwrl_5KP2.pdb: