Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLWKHGFKLLA-RMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHGVTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVVGIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL 4N69 Chain:B ((100-255)) -----------RERDPACVSYSHCLLNYKGFLACQAHRVAHLLWRQSRRPLALALHSRIANVFA-VDIHPAARIGKGILFDHATGVVVGETAVIGNNVSILHHVTLGGTGKVGGDRHPKIGDGVLIGAGATILGNIKIGEGAKVGAGSVVLIDVPPRTTAVGNPARLV--------------------------------------

General information: TITO was launched using: RESULT: Template: 4N69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 819 -29477 -35.99 -190.17 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -35.99 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_4N69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N69-query.scw PDB file : Tito_Scwrl_4N69.pdb: