Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHCLMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIK---VLHGPTVTD----WGTESLLVQGPAGLVLDFYRMK 1JIF Chain:A ((4-121)) FLGAVPVLTAVDVPANVSFWVDTLGFEKDFGDRDFAGVRRGDIRLHISRTEHQ---IVADNTSAWIEVTDPDALHEEWARAVSTDYADTSGPAMTPVGESPAGREFAVRDPAGNCVHFTAG-

General information: TITO was launched using: RESULT: Template: 1JIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 -45031 -97.05 -405.68 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -97.05 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_1JIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JIF-query.scw PDB file : Tito_Scwrl_1JIF.pdb: