Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKA---QFGKHIIVSAIEHPAVKESALWL-KSQGFEVDFAPVDKKGLVDVEALAGLIR--HDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALLN-YPDIFVFSDEE-NFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR 4ISY Chain:C ((13-394)) -AYLDHAATTPMHPAAIEAMAAVQRT-IGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEF-GVPMHSDAIQAVGQLPL-DFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGTPDVASAVGMATAAQIAVDGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSA-------PSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVARARR--

General information: TITO was launched using: RESULT: Template: 4ISY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2232 5821 2.61 15.86 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : 2.61 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4ISY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ISY-query.scw PDB file : Tito_Scwrl_4ISY.pdb: