Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLLVIGCGGVAQVAISKICQDSE-TFTEIMIASRTKSKCDDLKAKLEGKTSTKIET--AALDADKVEE-VIALIGSYKPEAVLNVALPYQDLTIMDACLATGVHYIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSGFDPGVTSVFSAYALKHYFDE-----------------------IHYIDI--LDCNGGDH---GYPFATNFNPEINLREVSAPGSYWEDGKWVEVE------------------AMSIKREYDFP-QVGQKDMYLLHHEEIESLAKNIPGVK-RIRFFMTFGQSYLTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVKDGVEKTIYIYNVCDHQECYAE-VGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEELDPDPFMEALNEYGLPWVVVENPQMVD 2PH5 Chain:A ((13-438)) KNRFVILGFGCVGQALMPLIFEKFDIKPSQVTIIAAEGTK-VDVAQQYG------VSFKLQQITPQNYLEVIGSTLE--ENDFLIDVSIGISSLALIILCNQKGALYINAATEPWKE------------------RR--TNYSLREEVLRLKDKTQKTALITHGANPGLVSHFIKEALLN-IAKDNGLTINRPKNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKSPGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMVRSWTPTLGAFHGFLITHAETISLTNFLT-LKNGSELLYRPTVHYAYN---PCPDARLSIFELKSN---EWKPQNKNRLILNEIID-----GCDELGVLLMGNQRGAYWYGST---LSIQEARQIAPYNNATSLQVVASMISGIIWAIE--HPDEGIVEPEEVDHQYIIDIAKPYLGKVG---------

General information: TITO was launched using: RESULT: Template: 2PH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1888 -7441 -3.94 -20.50 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -3.94 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_2PH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PH5-query.scw PDB file : Tito_Scwrl_2PH5.pdb: