Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKKLQFIASCPQHGVLDHPPNECHITQEY---VAALLFKQLGNYGFDAQHIVHEHE-----KVEVSIDNHLLPLSITCQQTDHDGHLMCEISANPDEEQDWFEKIETQSIIRQLAQAVENSLKADHSFSAFEWKS 4CRI Chain:A ((27-145)) SFVGLRVVAKWSSNG----YFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVKGHRKESGELYYSIEK--EGQRKWYKRMA---VILSLEQGNR--LREQYGLG------

General information: TITO was launched using: RESULT: Template: 4CRI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 484 13262 27.40 120.56 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 27.40 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_4CRI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CRI-query.scw PDB file : Tito_Scwrl_4CRI.pdb: