Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKKPVPKIYR--TTNWSSYNQALIKR--------GNISIWFDPKTQWYAQPQGKHGRNQT---------YSDAAIQCCLMIKSLFRLSLRMVTGFAQSLIKLCGLNWTAPDYSTLCRRQKRID--IAISYQQSRDGLHLLVDSTGLKFLGE----GEWKRKKHQPAYRRQWRKLHIGIDAKTLQIRAVQLTTNNVS--DSQVLGDLLDQIPLDERIDSVYTDGAYDTKHCRQV-ILDRDAHAVIPPRKNAKPWKDKK-------------------LRSLARNELLKTVKRLGRSLWKKWSGYHRRSLVETKMHCIK 1L9V Chain:A ((1-313)) MAELACFCYPHLENDSYKFIPFNNLAIKAMLTAKVDKKDMDKFYDSIIYGIAPPPQFKKRYNTNDNSRGMNFETIMFTKVAMLICEALN----SLKVTQANVSNVLSRVVSIRHLENLVIRKENPQDILFHSKDLLLKSTLIAIGQSKEIETTITAEGGEIVFQNAAFTMWKLTYLEHQLMPILDQNFIEYKVTLNEDKPISDVHVKELVAELRWQYNKFAVITHG---KGHYRIVKYSSVANHADRVYATFKSNVKTGVNNDFNLLDQRIIWQNWYAFTSSMKQGNTLDVCKRLLFQKMKPEKNPFKGLSTDRKMDEVS

General information: TITO was launched using: RESULT: Template: 1L9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 17502 14.65 66.55 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 14.65 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_1L9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L9V-query.scw PDB file : Tito_Scwrl_1L9V.pdb: