Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIQGKHFVITGGGSGLGAATAEYLVKQGASVTLVDMNVEAGEQQAKHLGPKAGFVKLDVTDEATAEQFFKDVLVKHGHLHGLVNCAGIGPSAKVV--GREGVHDLGLFAKTLNINVTGTFNMLRFAADAMSKNTVEEGEEDRGIIVNTASVAAFDGQIGQAAYSASKGAIVAMTLPIARELARHAIRIMTIAPGIMETPMLKGMPQNVQDALGQMVPYPSRLGKPEEFARLVAHIAENSYLNGEVIRLDGAIRMAAK 1E6W Chain:D ((6-258)) -SVKGLVAVITGGASGLGLSTAKRLVGQGATAVLLDVPNSEGETEAKKLGGNCIFAPANVTSEKEVQAALTLAKEKFGRIDVAVNCAGIAVAIKTYHEKKNQVHTLEDFQRVINVNLIGTFNVIRLVAGVMGQNEPDQGGQ-RGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVVTIAPGLFATPLLTTLPDKVRNFLASQVPFPSRLGDPAEYAHLVQMVIENPFLNGEVIRLDGAIRM---

General information: TITO was launched using: RESULT: Template: 1E6W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1386 -74445 -53.71 -296.59 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -53.71 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_1E6W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E6W-query.scw PDB file : Tito_Scwrl_1E6W.pdb: