Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVTAIVKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEIAISDEMVDAVIESITRVASTGKIGDGKIFLTNLEQVIRIRTGETGPDAV 3LF0 Chain:B ((3-114)) MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQ-GHTEVYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRAARTGKIGDGKVWVSPVDTIVRVRTGERGHDAL

General information: TITO was launched using: RESULT: Template: 3LF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 433 -64025 -147.86 -576.80 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -147.86 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_3LF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LF0-query.scw PDB file : Tito_Scwrl_3LF0.pdb: