Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATPFWYNTALHLLKPFYRWRIKRRAESLELYQQECLERFGPFEAPKNVKAIWFHAVSVGETNAAQPLIEYYLKLGQPVLVTNTTKTGQARAKSLFLKEPYLDLFQAVYLP-----------VDQKPLLKKFFELYQPKLLALVETELWPNLIDQAKLQHVPCLLLNARLSEKSAKGYGKVSGLTAGMLKQLDWVLAQDSATRQRYVELGLDEHKSQVVGNIKFDIHAPEAFIKQAAQLRQQWYLENRQVVTIASTH-AP-EEQQILEAFAPYLNSDRELVCIVVPRHPERFDEVFEICQNLNLITHRRSMGQSIHASTQVYLADS--MGELWLWYALSQVCFVGGSLNEPGGGHNILEPMVLNVPTVVGPRYFNFQTIVDEFIDENAVLIAQDAQQVVDIWLACLAEPEATEQLVAQAHKVLQRNQG---SLQKHIGVINRYLAEKS 4N9W Chain:A ((18-368)) ---------------------------------------------------------PGGVQSHVLQLAEVLRDAGHEVSVLAPASPHVKLPD-YVV-----SGGKAVPIPYNGSVARLRFGPATHRKVKKWIAEGDFDVLHIHEPNAPSLSMLALQAAEGPIVATFHTSTTKSLTLS-VFQGILRPYHEKIIGRIAVS----------------AVEIPNGVDVASFADAPL------LDGYPREGRTVLFLGRYDEPRKGMAVLLAALPKLVARFPDVEILIVGRGDE--DELREQAGDL--A-------------GHLRFLGQVDDATKASAMRSADVYCAPHLGGE-SFGIVLVEAMAAGTAVV-ASDLDAFRRVLAD-GDAGRLVPVDDADGMAAALIGILEDDQLRAGYVARASERVH-RYDWSVVSAQIMRVYETVS----

General information: TITO was launched using: RESULT: Template: 4N9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1568 -38444 -24.52 -120.51 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -24.52 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4N9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N9W-query.scw PDB file : Tito_Scwrl_4N9W.pdb: