Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQSGGFLVMCPFCGRSKGGLVCGYCGWVSKDKKFRFVVVKNG------------- 2EPR Chain:A ((1-48)) GSSGSS-------GRTRKQVACEICGKIFRDVYHLNRHKLSHSGEKPYSSGPSSG

General information: TITO was launched using: RESULT: Template: 2EPR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 54 -6955 -128.80 -198.71 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -128.80 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_2EPR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EPR-query.scw PDB file : Tito_Scwrl_2EPR.pdb: