Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQDRSVTELVN--YDRAFIVLHGRGGEDGQIQGVLEWLNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRIITKETDLDS----VIAELGLPVIIKPVHEGSSVGMSKVEKAEDFAAAIEKATQHDAVVMAEKWITGR-EFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCLRAFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKATKAVGYSFDELCVAILEQTLEGTA 4EG0 Chain:B ((12-315)) -----RFGKVAVLFGGESAEREVSLTSGRLVLQGLRDAGIDAHPFDPAERPLSALKDEGFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGVPTPPFETVMRGDDYAARATDIVAKLGLPLFVKPAS------VLKVKTADALPAALSEA--HDKIVIVEKSIEGGGEYTACIAGDLDLPLIKIVPAGE--FYD---------TQYLIPCGLPAEQETELKRIARRAFDVLGCTDWGRADFMLDAAGNAYFLEVNTAPGMTDHSLPPKAARSIGIGYSELVVKVLSLTL----

General information: TITO was launched using: RESULT: Template: 4EG0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1518 -514 -0.34 -1.83 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -0.34 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4EG0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EG0-query.scw PDB file : Tito_Scwrl_4EG0.pdb: