Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMVKGLKNKVALVTGAAQGIGRRIALRLAQEGAHIALVDMKQDRLNDVQQEIQALGVNASTFIADVSDREQVYAAINHAEQTLGGFDVMINNAGIAQVQAIADITPDEFQKIVDINIGGVLWGIQAAAAKFKQREHKGKIINACSIADHDGFALLGIYSATKFAVRALTQAAAKEYASHGITVNGYCPGIVGTDMWVEIDKRFAEITSAAIGETYKKYVEGIALGRAQTPDDVAALVAFLASEDSDYITGQSILTDGGIVYR 3WDS Chain:D ((10-260)) ------LSGRKAIVTGGSKGIGAAIARALDKAGATVAIADLD---VMAAQAVVAGLENGGFAVEVDVTKRASVDAAMQKAIDALGGFDLLCANAGVSTMRPAVDITDEEWDFNFDVNARGVFLANQIACRHFLASNTKGVIVNTASLAAKVGAPLLAHYSASKFAVFGWTQALAREMAPKNIRVNCVCPGFVKTAMQEREIIWEAELRGMTPEAVRAEYVSLTPLGRIEEPEDVADVVVFLASDAARFMTGQGINVTGGV---

General information: TITO was launched using: RESULT: Template: 3WDS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1472 -152131 -103.35 -606.10 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -103.35 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3WDS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WDS-query.scw PDB file : Tito_Scwrl_3WDS.pdb: