Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHA----FAEKTGVDLNYQKRLAPLDGFESSMRS-FFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTLWMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVI--ANRTLARAEQLVDDLKTAVPQAQLQAISLNDLEGDFDIVINATSASLSGDALQLPEKLK-----FKYAYEMAYGKPSSFLDQAKQRNVPYAEGFGMLVGQAIEAFSIWNGVRPQLKDFL 4FQ8 Chain:B ((4-246)) --KSFGVFGNPIKHSKSPLIHNACFLTFQKELRFLGHYHPILLPL---ESHIKSEFLHLGLSGANVTLPFKERAFQVCDKIKGIALECGAVNTLVLENDELVGYNTDALGFYLSLK--QKNYQNA--LILGAGGSAKALACELKKQGLQVSVLNRSSRGLDFFQRLGCDCFMEPPKSA------------FDLIINATSASLHNELPLNKEVLKGYFKEGKLAYDLAAGFLTPFLSLAKELKTPFQDGKDMLIYQAALSFEKFS----------

General information: TITO was launched using: RESULT: Template: 4FQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1268 11866 9.36 51.37 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 9.36 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_4FQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FQ8-query.scw PDB file : Tito_Scwrl_4FQ8.pdb: