Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEKGVETMKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKKYLT----NEKNITIIHTDEKINSDD-------------EPVKAIRRKKTASMVLAAQAVKNGEADAVFSAG--NTGALLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTEATKGSE---VTKKAYELLQNETSL-NFIGNVEAR--------------ELLNGVADVVVTDGF-TGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 1XCO Chain:D ((22-328)) -------DVKIVFPEGL----DERILEAVSKLAGNK-VLNPIVIGNENEIQAKAKELNLTLGGVKIYDPHTYEGMEDLVQAFVERRKGKATEEQAR-KALLDENYFGTMLVYKGLADGLVSGAAHSTADTVRPALQIIKTKEGVKKTSGVFIMARG-E--EQYVFADCAINIAPDSQDLAEIAIESANTAKM-FDI-EPRVAMLSFSTKGSAKSDETEKVADAVKIAKEKAPELTLDGEFQFDAAFVPSVAEKKAPDSEIKGDANVFVFPSLEAGNIGYKIAQ------------RLG-------------------------NFEAVGPILQGLNMPVNDLSRGCNAEDVYNLALITAAQA-----------------

General information: TITO was launched using: RESULT: Template: 1XCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1390 6164 4.43 22.91 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : 4.43 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_1XCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XCO-query.scw PDB file : Tito_Scwrl_1XCO.pdb: