Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAI-----VKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKI-----KGRKIYLSPYT 5HI8 Chain:A ((1-136)) NSMIDKFCDWFEGEFDNWTQAASNPTKWAHIIVKHEKISEYKYHTSSRYSYMDKPYREQTVDIEYVCPELIIVH-----NPACDIIFKWTGIY--FEGESEPDCQWNGQPLDSKARLYADEYHTWDVGYWEGSEGFFHFKKNV

General information: TITO was launched using: RESULT: Template: 5HI8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 499 3942 7.90 31.28 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 7.90 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_5HI8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HI8-query.scw PDB file : Tito_Scwrl_5HI8.pdb: